abstract：:The cloning and expression of toxic proteins in bacteria have posed a great challenge because of the leaky expression in inducible expression systems. Using artificial gene synthesis and clone screening methods, we identified a mutant T5 promoter, which significantly reduced leaky expression of lac operator. The mutan...

journal_title：World journal of microbiology & biotechnology

authors： Guo J,Jia R

更新日期：2014-05-01 00:00:00

abstract：:Biosurfactant-producing microorganisms inhabiting oil reservoirs are of great potential in industrial applications. Yet, till now, the knowledge about the structure and physicochemical property of their metabolites are still limited. The aim of this study was to purify and structurally characterize the biosurfactant f...

journal_title：World journal of microbiology & biotechnology

authors： Liu JF,Wu G,Yang SZ,Mu BZ

更新日期：2014-05-01 00:00:00

abstract：:Thiazolidinedione (TD) derivatives have been found to have an algicidal effect on harmful algal bloom microalgae. In this study, 75 TD derivatives were synthesized and analyzed for algicidal activity. Among these synthetic TDs, 18 TD derivatives showed specific algicidal activity on two strains belonging to the classe...

journal_title：World journal of microbiology & biotechnology

authors： Wu Y,Lee Y,Jung SG,Kim M,Eom CY,Kim SW,Cho H,Jin E

更新日期：2014-05-01 00:00:00

abstract：:In the present work, novel heterotrophic nitrifying and aerobic denitrifying bacteria have been isolated from greenwater system of coastal aquaculture. Based on the 16S rRNA gene, FAME analysis and biochemical test, the isolates have been identified as Pseudomonas aeruginosa and Achromobacter sp. These have been named...

journal_title：World journal of microbiology & biotechnology

authors： Kathiravan V,Krishnani KK

更新日期：2014-04-01 00:00:00

abstract：:Nitrification plays a significant role in the global nitrogen cycle, and this concept has been challenged with the discovery of ammonia-oxidizing archaea (AOA) in the environment. In this paper, the vertical variations of the diversity and abundance of AOA in the hyporheic zone of the Fuyang River in North China were ...

journal_title：World journal of microbiology & biotechnology

authors： Wang Z,Wang Z,Huang C,Pei Y

更新日期：2014-04-01 00:00:00

abstract：:With rapid urbanization, anthropogenic activities are increasingly influencing the natural environment of the Bohai Bay. In this study, the composition and variation of bacterial and nirS-harboring bacterial communities in the coastal zone sediments of the Bohai Gulf were analyzed using PCR-based clone libraries. A to...

journal_title：World journal of microbiology & biotechnology

authors： Guan X,Zhu L,Li Y,Xie Y,Zhao M,Luo X

更新日期：2014-04-01 00:00:00

abstract：:The present study was conducted for optimization of in vitro substrates under aseptic conditions for interaction of Piriformospora indica with the medicinal plant Coleus forskohlii. It aims to test the effects of different substrates on P. indica colonization as well as growth parameters of the in vitro raised C. fors...

journal_title：World journal of microbiology & biotechnology

authors： Das A,Tripathi S,Varma A

更新日期：2014-03-01 00:00:00

abstract：:A recently described marine gliding bacterium Rapidithrix thailandica strain TISTR 1741 was isolated from biofilm specimen collected from the Andaman Sea in Thailand. Four liters fermentation broth of R. thailandica TISTR 1741 cultivated in VY/2 medium were extracted with methanol to yield a novel amino phenyl pyrroli...

journal_title：World journal of microbiology & biotechnology

authors： Sangnoi Y,Plubrukarn A,Arunpairojana V,Kanjana-Opas A

更新日期：2014-03-01 00:00:00

abstract：:Alicyclobacillus acidoterrestris is one of the most spoilage-causing bacteria in fruit juices. Control of A. acidoterrestris in fruit juices by bificin C6165 (Pei et al. in J Appl Microbiol 114(5):1273-1284, 2013), a bacteriocin produced by Bifidobacterium animalis subsp. animalis CICC 6165, was described in this stud...

journal_title：World journal of microbiology & biotechnology

authors： Pei J,Yue T,Yuan Y

更新日期：2014-03-01 00:00:00

abstract：:Aspergillus oryzae aminohydrolase free acid phosphodiesterase catalyzes nicotinamide adenine dinucleotide to deamino-NAD and ammonia. The enzyme was purified to homogeneity by a combination of acetone precipitation, anion exchange chromatography and gel filtration chromatography. The enzyme was purified 230.5 fold. So...

journal_title：World journal of microbiology & biotechnology

authors： Ali TH,Ali NH,Haroun BM,Tantawy AE

更新日期：2014-03-01 00:00:00

abstract：:The aim of this study was to investigate the space mutagenesis of genetically engineered bacteria expressing recombinant human interferon α1b. The genetically engineered bacteria expressing the recombinant interferon α1b were sent into outer space on the Chinese Shenzhou VIII spacecraft. After the 17 day space flight,...

journal_title：World journal of microbiology & biotechnology

authors： Wang J,Liu C,Liu J,Fang X,Xu C,Guo Y,Chang D,Su L

更新日期：2014-03-01 00:00:00

abstract：:One strain of algicidal bacterium, named as S088, was isolated from the intestine of healthy sea cucumbers (Stichopus horrens) in the South China Sea. Based on the analysis of its biochemical characteristics and 16S rDNA gene sequence, S088 was identified as Bowmanella denitrificans. Importantly, the algicidal activit...

journal_title：World journal of microbiology & biotechnology

authors： Jiang X,Ren C,Hu C,Zhao Z

更新日期：2014-02-01 00:00:00

abstract：:The effects of different alkyl chain lengths of ionic liquids 1-ethyl-, 1-butyl- and 1-hexyl-3-methylimidazolium chloride, on the catalytic activity, thermal stability and deactivation kinetics of horseradish peroxidase were studied in the temperature range of 45-85 °C. The presence of 1-ethyl- and 1-butyl-ionic liqui...

journal_title：World journal of microbiology & biotechnology

authors： Machado MF,Queirós RP,Santos MD,Fidalgo LG,Delgadillo I,Saraiva JA

更新日期：2014-02-01 00:00:00

abstract：:In the present study, the biosynthesis of silver nanoparticles (AgNPs) using Neurospora intermedia, as a new non-pathogenic fungus was investigated. For determination of biomass harvesting time, the effect of fungal incubation period on nanoparticle formation was investigated using UV-visible spectroscopy. Then, AgNPs...

journal_title：World journal of microbiology & biotechnology

authors： Hamedi S,Shojaosadati SA,Shokrollahzadeh S,Hashemi-Najafabadi S

更新日期：2014-02-01 00:00:00

abstract：:Studies were carried out to screen and identify strains that are able to directly produce ferulic oligosaccharides (FOs) from wheat bran (WB). The inducement and distribution of hemicellulases from strain 2012, which was identified as a non-melanin secreting strain of Aureobasidium pullulans (A. pullulans), were also ...

journal_title：World journal of microbiology & biotechnology

authors： Yu XH,Gu ZX

更新日期：2014-02-01 00:00:00

abstract：:The induction of laccase isoforms in Trametes versicolor HEMIM-9 by aqueous extracts (AE) from softwood and hardwood was studied. Samples of sawdust of Pinus sp., Cedrela sp., and Quercus sp. were boiled in water to obtain AE. Different volumes of each AE were added to fungal cultures to determine the amount of AE nee...

journal_title：World journal of microbiology & biotechnology

authors： Bertrand B,Martínez-Morales F,Tinoco R,Rojas-Trejo S,Serrano-Carreón L,Trejo-Hernández MR

更新日期：2014-01-01 00:00:00

abstract：:Elicitation is an important strategy to improve production of secondary metabolites in vitro. Artemisinic acid was studied as a novel elicitor to enhance the yield of terpenoid indole alkaloids in the present paper. Our results demonstrated that the concentrations of vindoline and vinblastine were increased by sixfold...

journal_title：World journal of microbiology & biotechnology

authors： Liu J,Zhu J,Tang L,Wen W,Lv S,Yu R

更新日期：2014-01-01 00:00:00

abstract：:The gastrointestinal microbiota plays a crucial role in the health and disease of the host through its impact on nutrition. Gut microbial composition is related to different diets, but an association of microbiota with different diets in infant has not yet been shown. In this work, we compared the fecal microbiota of ...

journal_title：World journal of microbiology & biotechnology

authors： Fan W,Huo G,Li X,Yang L,Duan C,Wang T,Chen J

更新日期：2013-12-01 00:00:00

abstract：:Trehalose synthase (TreS) catalyzes the reversible interconversion of maltose and trehalose. A novel treS gene with a length of 3,369 bp, encoding a protein of 1,122 amino acid residues with a predicted molecular mass of 126 kDa, was cloned from a marine Pseudomonas sp. P8005 (CCTCC: M2010298) and expressed in Escheri...

journal_title：World journal of microbiology & biotechnology

authors： Gao Y,Xi Y,Lu XL,Zheng H,Hu B,Liu XY,Jiao BH

更新日期：2013-11-01 00:00:00

abstract：:Trichoderma asperellum parasitizes a large variety of phytopathogenic fungi. The mycoparasitic activity of T. asperellum depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall and proteases which are a group of enzymes capable of degrading proteins from host. In this stud...

journal_title：World journal of microbiology & biotechnology

authors： Yang X,Cong H,Song J,Zhang J

更新日期：2013-11-01 00:00:00

abstract：:Olive trees play an important role in cultural, ecological, environmental and social fields, constituting in large part the Mediterranean landscape. In Tuscany, an important economic activity is based on olive. Unfortunately, the Verticillium wilt affects this species and causes vascular disease. In the present study,...

journal_title：World journal of microbiology & biotechnology

authors： Ceccherini MT,Luchi N,Pantani OL,Ascher J,Capretti P,Pietramellara G

更新日期：2013-10-01 00:00:00

abstract：:Andrimid, a known non-ribosomal pseudo-peptide antibiotic, was isolated from a psychrotolerant Serratia proteamaculans strain. The antibiotic peptide was produced at low temperature (8 °C) in a 7.5 l BIOFLO 101 bioreactor under batch culture mode. Andrimid activity from S. proteamaculans culture was only detected at 2...

journal_title：World journal of microbiology & biotechnology

authors： Sánchez LA,Sierra MG,Siñeriz F,Delgado O

更新日期：2013-10-01 00:00:00

abstract：:The present investigation entails the immobilisation and characterisation of Escherichia coli MO1-derived carbonic anhydrase (CA) and its influence on the transformation of CO₂ to CaCO₃. CA was purified from MO1 using a combination of Sephadex G-75 and DEAE cellulose column chromatography, resulting in 4.64-fold purif...

journal_title：World journal of microbiology & biotechnology

authors： Oviya M,Sukumaran V,Giri SS

更新日期：2013-10-01 00:00:00

abstract：:Valsa mali var. mali (Vmm), is the predominant species of apple valsa canker in China. Modern analysis of genes involved in virulence or pathogenicity usually implicate gene expression analysis most often performed using real-time quantitative polymerase chain reaction (RT-qPCR). However, for relative gene expression ...

journal_title：World journal of microbiology & biotechnology

authors： Yin Z,Ke X,Huang D,Gao X,Voegele RT,Kang Z,Huang L

更新日期：2013-09-01 00:00:00

abstract：:This study was conducted to evaluate the effects of Bacillus subtilis SC02 supplement on water quality, microbial community diversity and structure in a grass carp (Ctenopharyngodon idellus) culture. Our selected strain, B. subtilis SC02, significantly reduced ammonia, nitrite and total nitrogen levels in water over a...

journal_title：World journal of microbiology & biotechnology

authors： Zhang X,Fu L,Deng B,Liang Q,Zheng J,Sun J,Zhu H,Peng L,Wang Y,Wenying S,Li W

更新日期：2013-09-01 00:00:00

abstract：:A novel 1,170 bp β-galactosidase gene sequence from Halomonas sp. S62 (BGalH) was identified through whole genome sequencing and was submitted to GenBank (Accession No. JQ337961). The BGalH gene was heterologously expressed in Escherichia coli BL21(DE3) cells, and the enzymatic properties of recombinant BGalH were stu...

journal_title：World journal of microbiology & biotechnology

authors： Wang GX,Gao Y,Hu B,Lu XL,Liu XY,Jiao BH

更新日期：2013-08-01 00:00:00

abstract：:Common reed (Phragmites australis) and narrow-leaved cattail (Typha angustifolia L.) are two plant species used widely in artificial wetlands constructed to treat wastewater. In this study, the community structure and diversity of root-associated bacteria of common reed and narrow-leaved cattail growing in the Beijing...

journal_title：World journal of microbiology & biotechnology

authors： Li YH,Zhu JN,Liu QF,Liu Y,Liu M,Liu L,Zhang Q

更新日期：2013-08-01 00:00:00

abstract：:A novel Lactobacillus panis PM1 isolate was found to be capable of converting glycerol to 1,3-propanediol (1,3-PDO), an increasingly valuable commodity chemical. In this study the effects of various process parameters, including glucose and glycerol concentrations, inoculum size, temperature, aeration, pH, and carbon ...

journal_title：World journal of microbiology & biotechnology

authors： Grahame DA,Kang TS,Khan NH,Tanaka T

更新日期：2013-07-01 00:00:00

abstract：:This study aimed to inhibit the growth of Escherichia coli and Clostridium tyrobutyricum, common bacteria responsible for early and late cheese blowing defects respectively, by using novel aqueous extracts obtained by dynamic solid-liquid extraction and essential oils obtained by solvent free microwave extraction from...

journal_title：World journal of microbiology & biotechnology

authors： Librán CM,Moro A,Zalacain A,Molina A,Carmona M,Berruga MI

更新日期：2013-07-01 00:00:00

abstract：:The use of indigenous bacterial strains is a valuable bioremediation strategy for cleaning the environment from hydrocarbon pollutants. The isolation and selection of hydrocarbon-degrading bacteria is therefore crucial for obtaining the most promising strains for site decontamination. Two different media, a minimal me...

journal_title：World journal of microbiology & biotechnology

authors： Bučková M,Puškarová A,Chovanová K,Kraková L,Ferianc P,Pangallo D

更新日期：2013-06-01 00:00:00

abstract：:Biosurfactant production through a fermentation process involving the biodegradation of soybean oil refining wastes was studied. Pseudomonas aeruginosa MR01 was able to produce extracellular biosurfactant when it was cultured in three soybean oil refinement wastes; acid oil, deodorizer distillate and soapstock, at dif...

journal_title：World journal of microbiology & biotechnology

authors： Partovi M,Lotfabad TB,Roostaazad R,Bahmaei M,Tayyebi S

更新日期：2013-06-01 00:00:00

abstract：:Experiments were conducted to evaluate the efficacy of calliterpenone, a natural plant growth promoter from a shrub Callicarpa macrophylla Vahl., in enhancing the growth and yield promoting effects of plant growth promoting rhizobacteria (PGPRs), in menthol mint (Mentha arvensis L).This study is based on our previous ...

journal_title：World journal of microbiology & biotechnology

authors： Maji D,Barnawal D,Gupta A,King S,Singh AK,Kalra A

更新日期：2013-05-01 00:00:00

abstract：:This study investigated effects of wet and dry seasons on the bacterial community structure of the Three Gorges Reservoir by using denaturing gradient gel electrophoresis analysis of the PCR-amplified bacterial 16S rRNA gene. Bacterial diversity, as determined by the Shannon index, the Simpson's index, and the Richnes...

journal_title：World journal of microbiology & biotechnology

authors： Chen Z,Zhou Z,Peng X,Xiang H,Xiang S,Jiang Z

更新日期：2013-05-01 00:00:00

abstract：:Mangrove fungi are vastly unexplored for enzymes with industrial application. This study aimed to assess the biocatalytic activity of mangrove fungal xylanases on recycled paper pulp. Forty-four mangrove fungal (MF) isolates were initially screened for xylanolytic activity in minimal medium with corn cob xylan as the ...

journal_title：World journal of microbiology & biotechnology

authors： Torres JM,Dela Cruz TE

更新日期：2013-04-01 00:00:00

abstract：:The incidence of fluoroquinolone-resistant Shigella strains has risen rapidly, presumably in response to ciprofloxacin antibiotic stress. Understanding the molecular mechanisms underlying this resistance phenotype is critical to developing novel and effective therapeutic strategies. In this study, the frequency of cip...

journal_title：World journal of microbiology & biotechnology

authors： Pu XY,Zhang Q,Pan JC,Shen Z,Zhang W

更新日期：2013-02-01 00:00:00

abstract：:The bioleachings of chalcopyrite ore were compared after inoculating different cultures enriched from the original acid mine drainage sample. The results showed that the higher bioleaching performance was achieved for inoculation with the enrichment D (0.5 % S, 2 % iron and 1 % chalcopyrite) compared to other enrichme...

journal_title：World journal of microbiology & biotechnology

authors： Xia L,Uribe P,Liu X,Yu C,Chai L,Liu J,Qiu W,Qiu G

更新日期：2013-02-01 00:00:00

abstract：:Bacteria associated with eight field-collected and five cultured soft corals of Briareum sp., Sinularia sp., Sarcophyton sp., Nephtheidae sp., and Lobophytum sp. were screened for their abilities in producing antimicrobial metabolites. Field-collected coral samples were collected from Nanwan Bay in southern Taiwan. Cu...

journal_title：World journal of microbiology & biotechnology

authors： Chen YH,Kuo J,Sung PJ,Chang YC,Lu MC,Wong TY,Liu JK,Weng CF,Twan WH,Kuo FW

更新日期：2012-12-01 00:00:00

abstract：:The volatile compounds produced by Debaryomyces hansenii NRRL Y-7426 during the fermentation of detoxified concentrated distilled grape marc hemicellulosic hydrolysates was analysed by GC-MS. Thirty-five compounds corresponding to ten groups of volatile compounds: terpenes, higher alcohols, C₆ alcohols, aldehydes, vol...

journal_title：World journal of microbiology & biotechnology

authors： Salgado JM,González-Barreiro C,Rodríguez-Solana R,Simal-Gándara J,Domínguez JM,Cortés S

更新日期：2012-11-01 00:00:00

abstract：:Bacteria are crucial components in lake sediments and play important role in various environmental processes. Urban lakes in the densely populated cities are often small, shallow, highly artificial and hypereutrophic compared to rural and natural lakes and have been overlooked for a long time. In the present study, ba...

journal_title：World journal of microbiology & biotechnology

authors： Zhao D,Huang R,Zeng J,Yan W,Wang J,Ma T,Wang M,Wu QL

更新日期：2012-11-01 00:00:00

abstract：:Oxalic acid plays major role in the pathogenesis by Sclerotinia sclerotiorum; it lowers the pH of nearby environment and creates the favorable condition for the infection. In this study we examined the degradation of oxalic acid through oxalate oxidase and biocontrol of Sclerotinia sclerotiorum. A survey was conducted...

journal_title：World journal of microbiology & biotechnology

authors： Yadav S,Srivastava AK,Singh DP,Arora DK

更新日期：2012-11-01 00:00:00